Prokaryotes



Hi and welcome, the last class of microbiology, Dr Wan told us to sit in our group and discuss what we want to take  microbe which in prokaryote. Our group pick bacteria, which is Propionibacterium acnes. So basically we must find platform to share our microbe. So we pick Testeach. There we find infomation then put it there. Some information that we find are about it classification, morphology, and etc about it. Below are some information that I can share about our microbe.

Kingdom:Bacteria
Phylum:Actinobacteria
Order:Actinomycetales
Family:Propionibacteriaceae
Genus:Propionibacterium
Species:P. acnes

MICROSCOPIC APPEARANCE

Gram Stain:Gram-positive.
Morphology:Pleomorphic, branched and unbranched rods, coccoid forms, or bifid, but they are not filamentous. Cells are often "club-shaped" with one end rounded and the other tapered. Cells occurs in singly, in pairs or short chains, in "V" or "Y" configurations, or in clumps with a "Chinese character" arrangement.
Size:0.5-0.8 micrometers by 1.0-5.0 micrometers.
Motility:Non-motile.
Capsules:None.
Spores:None.
Other:Readily confused with some species of Corynebacterium or Clostridium .

MACROSCOPIC APPEARANCE

On Blood Agar, colonies appear convex, semi-opaque, and glistening. Colonies have been seen pigmented in an array of colors from white to red.

KEY BIOCHEMICAL REACTIONS

  • Catalase-positive.
  • Indole-variable.

METABOLIC PROPERTIES

Some species are facultatively aerobic (acnes group) while others are variably aerotolerant (dairy group). Chemoorganotrophic; metabolism is primarily fermentative, with complex nutritional requirements. Hexoses (e.g. glucose) or lactate are fermented mainly to propionic and acetic acid.

HABITAT

Propionibacterium species are the predominant indigenous flora of the skin, but also can be recovered form the gastrointestinal tract, upper respiratory tract, especially the anterior nares, and the urogenital tract. Because of their presence on skin, Propionibacterium are often contaminants of specimens such as blood or other body fluids obtained through skin puncture.
Acnes group commonly found in the extraintestinal regions of humans.
Dairy group commonly found in cheese and other dairy products.

PATHOGENICITY

Found as secondary agents of infected sinuses, wounds, and abscesses in humans and other animals.
Specifically, the "acnes group" is associated with human skin and intestinal infections, while the "dairy group" is associated with cheese and dairy products. Various Propionibacterium spp., particularly P . acnes , have been implicated in infections of corneal ulcers, heart valves and prosthetic devices (e.g. artificial joints), and ventricular shunts. These infections often lead to osteomyelitis, bacteremia, endocarditis, and meningitis.

RECOMMENDED MEDIA

For culture:Tryptone Yeast Extract, Glucose Medium with 0.5% Tween ® 80.
For selective isolation:Yeast Extract, Sodium Lactate Medium (dairy).
For maintenance:Cooked Meat Medium for short-term maintenance and Lyophilization for long-term storage.


So that is that I can share to you guys. I will update next week. see yaa......

Comments

Post a Comment

Popular posts from this blog

THE FIRST EXPERIMENT

Image
             Hi everyone this blog is about the experiment or practical that i do with my group members. This blog will contain all the conclusion or summary of the experiment and also as reflection and the things that i newly found out. So last  Tuesday, my member and I do practical ambout the CULTURE TRANSFER TECHNIQUE. Which is that we just learn the technique of culturing and how to prevent it from contamination.              Above are the procedure on how to culturing. We got to transfer into three media which is  S. marcescens, broth culture "A" and broth culture "B". It transfer into  slant to broth transfer, broth to slant transfer and slant to agar deep transfer.          After we subculture it we wait for the next day to observe the results. The next day,  the result is observe below are the result that I obtain. S. marcescens to Nutrient Agar Deep S. marcescens  to Nutrient Broth S. marcescens  to Nutrient Agar Slant Broth culture "A" to Nutrien
Read more

First week in Microbiology class

Image
First day of class Microbiology (13/9/2017)            It is the first day class of microbiology. I'm slightly nervous and excited of what will we going through today. As our lecturer Dr. Wan came, she briefly introduces herself and she started to ask us question. She also told us to make 9 group which contain in a group 1 Malaysia. I'm in a group of 5 people. She throw a few question to us about microorganisms and the group of microorganisms. I feel flustered when suddenly Dr. Wan just ask us question without we learn anything but she said that it is her own ways to teach us which is the "Ask question style". She also said that once we go inside the the room, we also going inside the microbiology world.                    On that day, we also learn about the biggest bacteria in the world. It is Thimargarita namibiensis.  Is a gram negative coccoid Proteobacterium, found in ocean sediments of the contenential shelf of Namibia (Source: Wikipedi
Read more

Measurement & Motility of Microorganisms

Image
Hi and welcome to my blog. so our last practical class we did the experiment of measurement of microorganisms and the examination of microorganisms using hanging drop and wet mount.  The  hanging drop technique  is a well-established method for examining living, unstained, very small organisms. The traditional procedure employs a glass slide with a circular concavity in the centre into which a  drop  of fluid, containing the 'microorganisms', hangs from a coverslip. Meanwhile for   In a  wet mount , the specimen is placed in a drop of water or other liquid held between the slide and the cover slip by surface tension. This method is commonly used, for example, to view microscopic organisms that grow in pond water or other liquid media, especially when studying their movement and behavior. preparation of hanging-drop Preparing wet mount Meanwhile for the practical of measurement of microorganisms, we need ocular micrometer and stage micrometer. first we need to find the calibrati
Read more