Atmospheric oxygen requirements & Techniques for the Cultivation of Anaerobic Microorganisms

Hello guys, so this week experiment going to be the techniques for the cultivation of anaerobic  microorganisms and atmospheric oxygen requirements.

Microorganisms exhibit great diversity in their ability to use free oxygen for cellular respiration. These variation in Oxygen requirement reflect the differences in biooxidative enzyme systems present in the various species. Microorganisms can be classified into one of five major groups according to their O2 needs.


  • Aerobes
  • Microaerophiles
  • Obligate anaerobes
  • Aerotolerant anaerobes
  • Facultative anaerobes
Differentiating the aerobes and anaerobes , samples suspected of containing anaerobes need to be handled carefully and  transported promptly to a lab, where it are typically inoculated onto anaerobic blood agar plates and anaerobic broth, as well as onto MacConkey agar and aerobic blood plate. Growth on aerobic or anaerobic agars will determine oxygen requirements, while comparable growth on both aerobic and anaerobic media suggests a facultative anaerobe.


















Microorganisms differ in their abilities to use oxygen for cellular respiration. Respiration involves the oxidation of substrates for energy necessary to life. A substrate is oxidized when it loses a hydrogen ion and its electron. Since the hydrogen electron cannot remain free in the cell, it must immediately be picked up by an electron acceptor, which become reduced. Therefore, reduction means gaining the hydrogen electron. These termed oxidation-reduction (redox) reaction.

The following experiment uses fluid thioglycollate medium and the GasPak anaerobic system.








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