Serial Dilution - Agar Plate Procedure to Quantitate Viable Cells


Hi guys so this week experiment will going to be the Serial dilution. In this experiment, we will be able familiar and understand with the diverse methods used to determine the number of cells in a bacterial culture. The number of cells counted is calculated as follows:
Number of cells per mm= number of cells counted X dilution X 50,000

The factor of 50,000 is used in order to determine the cell count for 1ml:1ml=1000mm3=(50 times the chamber depth of 0.02) X 1000.

Next, we able to know how to determine quantitatively the number of viable cells in a bacterial culture. For this experiment we need to do the two procedure which are pour-plate and spread-plate. For the pour plate, we just need to pour 1ml of culture E. coli into empty petri dish and pour the molten agar. For the spread-plate, spread the 0.1 ml culture of E. coli into the surface of agar medium. Spread with the L-shaped bent glass rod.



so thats all thank you goodbye.





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